Webinar 31 de octubre 2019 Microheterogeneities are inherent features of monoclonal antibodies (mAbs) due to their susceptibility to chemical and enzymatic modifications. These heterogeneities impact both biotherapeutic efficacy and safety, and accordingly are a critical quality criterion.
Date: Thursday, October 31, 2019
Time: 01:30 PM Eastern Daylight Time
Registration link: www.waters.com/Oct31
Advancing mAb Charge Variant Characterization Using Native IEX-MS
Microheterogeneities are inherent features of monoclonal antibodies (mAbs) due to their susceptibility to chemical and enzymatic modifications. These heterogeneities impact both biotherapeutic efficacy and safety, and accordingly are a critical quality criterion. Many of these modifications generate slight differences in charge including oxidation, deamidation, lysine truncation, etc. These charge differences can be separated in the native state using ion exchange chromatography (IEX) and detected using mass spectrometry (MS). Herein, we present work showing the efficient analyses of mAb charge variants, at both the intact and subunit level, using the BioResolve Strong Cation Exchange (SCX) mAb column and IonHance CX-MS buffers.
